Brazilian ground beef authentication by multiplex polymerase chain reaction
نویسندگان
چکیده
منابع مشابه
Rfbo157 by Multiplex Polymerase Chain Reaction
A rapid method for detection of Escherichia coli O157: H7 using multiplex PCR was developed. Two oligonucleotide primer pairs were used for simultaneously detection of vt encoding verotoxin genes for virulence factor and rfbO157 encoding the O-antigen specific for E. coli O157: H7. Multiplex PCR generated two products of 215 bp and 420 bp for vt and rfbO157, respectively. Multiplex PCR detected...
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Escherichia coli O157:H7 is an important human pathogen causing haemorrhagic colitis, haemolyticuraemic syndrom and thrombotic thrombocytopenic purpura. In this study, 100 ground beef samples were collected randomly from beef markets in June 2004. For isolation of the bacteria, samples were firstly enriched in modified trypticase soy broth, followed by plating onto sorbitol MacConkey agar sup...
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ژورنال
عنوان ژورنال: Ciência Rural
سال: 2018
ISSN: 1678-4596
DOI: 10.1590/0103-8478cr20160574